ABSTRACT
Objective To determine content of Salidroside in Ligustri Lucidi Fructus at different growth stages and in different regions in Zhejiang Province by HPLC; To provide the basis for more rational, efficient extraction of Salidroside from Ligustri Lucidi Fructus.Methods The Kromasil C18 column (4.6 mm×150 mm, 5μm) was used with mobile phase of acetonitrile-water (10:90); flow rate was 1.0 mL/min; detection wavelength was 220 nm; column temperature was set at room temperature; injection volume was 20μL.Results Salidroside was in good linear range within 0.71–3.55μg (r=0.999 4). The recovery rate of Salidroside was 95.6%–98.6% and the average recovery rate was 96.88%, RSD was 1.32% (n=5).Conclusion The content of Salidroside in Ligustri Lucidi Fructus gradually decreased with the extending of growing season. The contents of Salidroside were different at different growth stages and in different regions.